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open access eISSN 2093-3673

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Original Article

Anat Cell Biol 2023; 56(4): 538-551

Published online December 31, 2023

https://doi.org/10.5115/acb.23.101

Copyright © Korean Association of ANATOMISTS.

A comparative study on the hepatoprotective effect of selenium-nanoparticles and dates flesh extract on carbon tetrachloride induced liver damage in albino rats

Ghada Nady Ouais1,2 , Doaa Mohamad Hassan3

1Department of Human Anatomy and Embryology, Faculty of Medicine, Cairo University, Cairo, 2Department of Human Anatomy and Embryology, Faculty of Medicine, Newgiza University, Giza, 3Department of Human Anatomy and Embryology, Faculty of Medicine, Beni-Suef University, Beni Suef, Egypt

Correspondence to:Doaa Mohamad Hassan
Department of Human Anatomy and Embryology, Faculty of Medicine, Beni-Suef University, Beni Suef 62511, Egypt
E-mail: doaa_eldary333@yahoo.com

Received: April 14, 2023; Revised: May 27, 2023; Accepted: June 30, 2023

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Exposure to environmental pollutants such as carbon tetrachloride (CCL4) causes liver damage. This study aimed to compare the ameliorative activity of the dates flesh extract (DFE) and selenium-nanoparticles (SeNPs) on CCL4-induced hepatotoxicity and if DFE could be a useful alternative supplement. Twenty-four male albino rats were enrolled and randomly divided into four equal groups (6 rats in each group): control group received only basal diet with no medications. Group II received CCL4 in a dose of 0.5 mg/kg intraperitoneal injection twice weekly for four weeks. Group III rats were pretreated with SeNPs in a dose of 2.5 mg/kg once a day orally three times/wk for four weeks alone then combined with the previously described dose of CCL4 for another four weeks. Group IV rats were pretreated with DFE in a dose of 8 ml of the aqueous extract/kg/d orally for four weeks alone then combined with the previously described dose of CCL4 for another four weeks. The liver damage was assessed by estimation of plasma concentration of albumin and enzymes activities of alanine aminotransferase and tissue genes expression. Liver oxidation levels were assessed by measuring the tissue concentration of the malondialdehyde, superoxide dismutase, and the total glutathione. Additionally, inflammatory mediators tumour necrosis factor--α and interleukin-6 were estimated. Detecting the liver’s cellular structural damage was done by histopathological and immunohistochemical examination. This study suggests that CCL4-induced liver damage in rats can be protected by administration whether the costly SeNPs or the economical DFE.

Keywords: Selenium-nanoparticles, Dates-flesh-extract, Hepatoprotection, Carbon tetrachloride

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