Anat Cell Biol 2022; 55(3): 320-329
Published online September 30, 2022
Copyright © Korean Association of ANATOMISTS.
1Department of Human Anatomy, Faculty of Biomedical Sciences, Kampala International University, Bushenyi, Uganda, 2Department of Human Anatomy, College of Medicine and Health Science, Ahmadu Bello University, Zaria, 3Department of Human Anatomy, Federal University Dutse, Jigawa, Nigeria, 4Department of Human Anatomy, College of Medicine and Pharmacy, University of Rwanda, Kigali, Rwanda, 5Infection Medicine, Deanery of Biomedical Sciences, College of Medicine and Veterinary Medicine, The University of Edinburgh, Edinburgh, United Kingdom
Correspondence to:Ibe Michael Usman
Department of Human Anatomy, Faculty of Biomedical Sciences, Kampala International University, Bushenyi 00256, Uganda
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
Aluminium exposure has been linked with developmental neurotoxicity in humans and experimental animals. The study aimed to evaluate the ameliorative effect of Tamarindus indica on the developing cerebellar cortex, neurobehavior, and immunohistochemistry of the cerebellar cortex following prenatal aluminum chloride (AlCl3) exposure. Pregnant timed Wistar rats were divided into 5 groups (n=4). Group I (negative control) was given distilled water, group II was treated with 200 mg/kg of AlCl3, group III were given 200 mg/kg of AlCl3 and 400 mg/kg of ethyl acetate leaf fraction of Tamarindus indica (EATI), group IV were given 200 mg/kg of AlCl3 and 800 mg/kg of EATI, and group V were treated with 200 mg/kg of AlCl3 s/c and 300 mg/kg of vitamin E for 14 days (prenatal day 7–21) via the oral route. Male pups (n=6) were randomly selected and taken for neurobehavioral studies, and humanely sacrificed via intraperitoneal injection of thiopental sodium. The cerebellum was removed, fixed and tissue processed for histological and immunohistochemical studies. The results revealed that prenatal AlCl3 exposure impacted neurodevelopment and neurobehaviour among exposed pups. Prenatal AlCl3 exposure was marked with delayed cytoarchitectural development of the cerebellar cortex and increased GFAP expression in the cerebellar cortex. On the other hand, treatment with EATI and vitamin E were marked with significant improvements. The present study therefore concluded treatment with EATI shows an ameliorative effect to prenatal AlCl3 exposure.
Keywords: Tamarindus indica, Glial fibrillary protein, Motor cordination, Aluminum exposure