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Original Article

Anat Cell Biol 2022; 55(2): 229-238

Published online June 30, 2022

https://doi.org/10.5115/acb.21.233

Copyright © Korean Association of ANATOMISTS.

The in vitro analysis of migration and polarity of blastema cells in the extracellular matrix derived from bovine mesenteric in the presence of fibronectin

Kamelia Kohannezhad1,2,* , Soroush Norouzi1,2,* , Maryam Tafazoli1,2 , Safoura Soleymani3 , Nasser Mahdavi Shahri1,2 , Amin Tavassoli1,3

1Department of Biology, Kavian Institute of Higher Education, Mashhad, 2Department of Biology, Faculty of Sciences, Ferdowsi University of Mashhad, Mashhad, 3Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

Correspondence to:Amin Tavassoli
Division of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad 9177948974, Iran
E-mail: amin.tavassoli@mail.um.ac.ir

*These authors contributed equally to this work.

Received: November 23, 2021; Revised: January 19, 2022; Accepted: February 28, 2022

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Cell migration is an essential process in embryonic development, wound healing, and pathological conditions. Our knowledge of cell migration is often based on the two dimentional evaluation of cell movement, which usually differs from what occurred in vivo. In this study, we investigated cellular migration from blastema tissue toward bovine decellularized mesentery tissue. In this regard, fibronectin (FN) was assessed to confirm cell migration. Therefore, we established a cell migration model using blastema cells migration toward the extracellular matrix derived from bovine mesenteric tissue. A physiochemical decellularization method was utilized based on freeze-thaw cycles and agitation in sodium dodecyl sulfate and Triton X-100 to remove cells from the extracellular matrix (ECM) of bovine mesenteric tissue. These types of matrices were assembled by the rings of blastema tissues originated from the of New Zealand rabbits pinna and cultured in a medium containing FN in different days in vitro, and then they are histologically evaluated, and the expression of the Tenascin C gene is analyzed. By means of tissue staining and after confirmation of the cell removal from mesenteric tissue, polarity, and migration of blastema cells was observed in the interaction site with this matrix. Also, the expression of the Tenascin C gene was assessed on days 15 and 21 following the cell culture process. The results showed that the three dimentional model of cellular migration of blastema cells along with the ECM could be a suitable model for investigating cell behaviors, such as polarity and cell migration in vitro.

Keywords: Extracellular matrix, Tissue engineering, Mesentery, Tenascin-C, Cell migration

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