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Original Article

Anat Cell Biol 2021; 54(2): 225-231

Published online June 30, 2021


Copyright © Korean Association of ANATOMISTS.

Comparison of decomposition rate of hind limbs of preserved mice with ethanol-glycerin and formaldehyde of advanced fixative solution

Amanda Natalie Wijaya1 , Ria Margiana2 , Sasanthy Kusumaningtyas2 , Deswaty Furqonita2

1Undergraduate Program of Medical Education, Faculty of Medicine, Universitas Indonesia, Jakarta, 2Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia

Correspondence to:Ria Margiana
Department of Anatomy, Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia
E-mail: ria.margiana11@gmail.com

Received: December 4, 2020; Revised: February 22, 2021; Accepted: February 22, 2021

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Learning anatomy in medical school is still closely based on the use of cadavers. The burial of preserved cadaver poses a problem, specifically, it contaminates the soil with formalin. Many studies have been conducted to find an alternative fixative to update or modify formalin usage. One of them is ethanol-glycerin (EG), which suggests promising results. Despite that fact, there has yet to be any research comparing the decomposition rate between EG and formalin. This study is conducted to compare the rate of decomposition between the two fixative solutions, EG and 4% formalin on the hind limb of mice. The mice were first preserved using a standard primary fixative solution which is 10% formalin, following that procedure is preservation using advanced fixative solution, EG or 4% formalin. Upon completing the preservation steps, the mice were buried for 6 weeks and observed weekly. The stages of decomposition were assessed semi-quantitatively depending on its appearance. The hind limbs of mice that were fixed with EG solution managed to reach the last stage of decomposition, dry & remains, while the 4% formalin group of mice still remained in the previous stage, advanced decay. It is concluded that the mice hind limbs that have been previously preserved with EG advanced fixative solution has a faster decomposition rate compared to 4% formalin.

Keywords: Burial, Glycerol, Mice, Preservation

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