Anat Cell Biol
Published online January 14, 2021
Copyright © Korean Association of ANATOMISTS.
1Department of Medical Laboratory Sciences, Faculty of Pharmacy, Jadara University, Irbid, 2Department of Biological Sciences, School of Science, The University of Jordan, Amman, Jordan, 3Department of Medical Laboratory Science, College of Science, Komar University of Science and Technology, Sulaymaniyah, Iraq, 4Department of Anatomy and Histology, School of Medicine, The University of Jordan, Amman, Jordan
Correspondence to:Alia Khwaldeh
Department of Medical Laboratory Sciences, Faculty of Pharmacy, Jadara University, Irbid 21110, Jordan
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
This study aims to evaluate the chemopreventive activity of two antioxidants (curcumin [CUM] and caffeic acid [CAF]), focusing on how these antioxidants could reduce cytotoxicity induced by short term secondhand exposure of waterpipe tobacco smoking. Forty-eight adult male BALB/c albino mice were equally divided into four groups. Antioxidants were delivered intraperitoneally, and the exposure to waterpipe smoking (WPS) was performed using a smoking machine. This experiment lasts for 14 consecutive days. Serum were collected from mice before dissection to quantify the activity of some liver enzymes, kidney function tests and proinflammatory cytokines. Lung, heart, and kidney were isolated and processed for light microscopy technique. Parallel treatment of CUM or CAF along with exposure to WPS showed less inflammation, less vacuolized, and more inflated alveoli, less deteriorations in cortex part of kidney, and less disintegration of cardiac myofibers in comparison to waterpipe only. Besides, CUM and CAF significantly reduced the activity of aspartate aminotransferase and proinflammatory cytokines. CUM and CAF were found to have anti-inflammatory and ameliorative effects against the cytotoxicity induced by exposure to waterpipe tobacco smoking, and CUM showed better chemopreventive activity than CAF.
Keywords: Antioxidants, Biochemical parameters, Histopathology, Hookah, Proinflammatory cytokines