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Anat Cell Biol

Published online December 7, 2020


Copyright © Korean Association of ANATOMISTS.

JEG-3 placental cells in toxicology studies: a promising tool to reveal pregnancy disorders

Elodie Olivier1 , Anaïs Wakx1 , Sophie Fouyet1 , MÉlody Dutot1,2 , Patrice Rat1

1UMR CNRS 8038 CiTCoM, Laboratoire de Chimie-Toxicologie Analytique et Cellulaire, Université de Paris, Faculté de Pharmacie de Paris, Paris,
2Recherche & Développement, YSLAB, Quimper, France

Correspondence to:Elodie Olivier
UMR CNRS 8038 CiTCoM, Laboratoire de Chimie-Toxicologie Analytique et Cellulaire, Université de Paris, Faculté de Pharmacie de Paris, 75006 Paris, France
E-mail: elodie.olivier@u-paris.fr

Received: September 2, 2020; Revised: October 16, 2020; Accepted: November 3, 2020

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.


Placental alterations are responsible for adverse pregnancy outcomes like preeclampsia and intrauterine growth restriction. And yet, placenta toxicology has not become a fully-fledged toxicology field. Because placenta is very often seen only as a barrier between the mother and the fetus, there is a lack and therefore a need for an experimental human model with technical recommendations to study placenta toxicology. In vitro approaches are recommended in experimental toxicology as they focus on a specific biological process and yield high-throughput screening methods. In the present study, we first established incubation conditions to preserve signatures of the human JEG-3 cell line identity while enabling toxicity detection. JEG-3 cells prepared in our incubation conditions were renamed JEG-Tox cells. As placental alterations are mainly triggered by uncontrolled apoptosis, we second used known apoptotic agents pregnant women are exposed to, to check that JEG-Tox cells can trigger apoptosis. Ethanol, bisphenol F, quinalphos, 4,4’-DDT, benzalkonium chloride, phenoxyethanol, propylparaben, and perfluorooctanic acid all induced chromatin condensation in JEG-Tox cells. Our incubation conditions allow JEG-Tox cells to keep placental cell identity and to respond to toxic chemicals. JEG-Tox cells are a pertinent model for placenta toxicology and could be used to better understand pregnancy alterations.

Keywords: Placenta, Toxicology, In vitro, Apoptosis, Hormones

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