Fig. 6. (A) Photomicrographs of the of CA3 region of the hippocampus control group showing compact layers of small granular and pyramidal cells most with vesicular nuclei. Each neuron features a large nucleolus and a rounded central vesicular nucleus (arrows). (B) I/R micrograph showed marked neuronal degeneration with PC, K, DN, CV, PV in the histoarchitecture of the hippocampus. (C) I/R+50 mg/kg MNC group showed preservation of pyramidal cells with few pyknotic and degenerating neurons and mild clumping of granular cells. (D, E) I/R-induced ischemic stroke & treatment with PTT (100/200 mg/kg) group, showing improvement in the histoarchitecture of the hippocampal pyramidal and granular cells at CA1 and CA3 region (H&E, ×250). (F) is a graph of the stereological estimate of the hippocampal CA3 pyramidal cell number across the group. n=5; mean±SEM, one-way ANOVA, Tukey’s post-hoc test. CA1, cornu ammonis 1; CA3, cornu ammonis 3; G, granular cells; K, karyorrhexis; PV, perineural vacuolation; DN, dark neuron; CV, cytoplasmic vacuolation; PC, pyknotic cell; I/R, ischemia/reperfusion; MNC, minocycline; PTT, phenolics fraction of Tetrapleura tetraptera. aP<0.05 when compared to the control group, bP<0.05 when compared to the I/R group.
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