Fig. 3. Histological preparation and cross-sectional morphometry of the recurrent laryngeal nerve. (A) Photomicrography of the right recurrent laryngeal nerve (RLN) superficially to the cricoid cartilage (CC) and deep to the cricopharyngeus muscle (CPM). Scale bar=100 µm. (B) Inset of the RLN showing the analyzed area of histology slides for epineurium (black line), and perineural space (yellow shadow). Note the presence of two vasa nervorum within the epineurium and nerve axons enveloped by the perineural epithelium. Scale bar=50 µm. (C) Cross-sectional area of the epineurium (I) and perineural space (II) from the right (R) and left (L) recurrent laryngeal nerves. R and L scores from the same subjects are connected in the plot. ns, non significant P>0.05.
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