Anatomy & Cell Biology

Table 2

Static characteristics table representing extracted data from included articles

	Ahmadvand Koohsari et al. [15] (2021)	Zhou et al. [16] (2020)	Torkaman et al. [17] (2017)	Donders et al. [18] (2015)	Mikaeili Agah et al. [19] (2014)	Liu et al. [9] (2013)
Country	Iran	China	Iran	Belgium	Iran	China
Experimental MS model	EAE using MOG_35-55	EAE using MOG_35-55	EAE using MOG_35-55	EAE using MOG_35-55	EAE using MOG_35-55	EAE using MOG_35-55
Animal strains	C57/BL6 mice	C57/BL6 mice	C57/BL6 mice	DA rats	C57/BL6 mice	C57/BL6 mice
Animal sex	Female	Female	Female	Female	Female	Female
Animal weight (g)	NR	NR	NR	NR	NR	NR
Animal age (wk)	6–8	6–8	6–8	8	6–8	6–7
Sample size (N/group)	10	10	5	4	5	20
Source of umbilical cord	Human	Human	Human	Human	Human	Human
Citation source of hUCMSCs isolation and culture procedures	Bai et al. [20]	NR	Amari et al. [21]	NR	NR	NR
hUCMSCs surface markers & immunophenotyping	CD45^-, CD73⁺, CD105⁺	CD11b, CD19, CD34, CD45, CD73, CD90, CD105, HLA-DR	HLA-I, HLA-DR, CD34, CD73, CD45, CD117, CD90, CD105, CD31, CD44, CD80, CD86, CD40, IgG1	HLA-DR-PE, CD45-FITC, CD34-PE, CD14-PE, CD19-PE, CD105-PE, CD90-FITC, CD40-FITC, CD73-PE, CD80-PE, CD86-FITC	CD90, CD105, CD45, CD31	CD3-APC, CD4-FITC, CD8-PE
hUCMSCs differentiation	Adipogenesis (using dexamethasone and insulin) & osteogenesis (using dexamethasone and ascorbic acid)	Not mentioned	Adipogenesis (using dexamethasone and indomethacin)	Not mentioned	Neurogenesis (using insulin transferrin selenium, fibronectin, FGF2, EGF, poly-l-ornithine, and PDGF)	Not mentioned
Main applied component	Extracellular vesicles derived from hUCMSCs	hUCMSCs	hUCMSCs	Co-culturing of WJ-MSCs and peripheral blood mononuclear cells	Differentiation of human Wharton’s jelly stem cells into OPCs	hUCMSCs
Route of material application	IV (tail vein)	IV (tail vein)	IV (tail vein)	IV (tail vein)	Intracerebroventricular injection	IV (tail vein)
Day of treatment post-immunization	9	14	3 & 11	28	7	15
Level of material application	50 μg of extracellular vesicles derived from hUCMSCs	10⁷ cells/ml	10⁶ cells/ml	2×10⁶ cells/ml	5×10⁵ cells/7.5 μl OPC medium	2×10⁷
Optimized (pre-treatment) with	None	IFN-γ (20 ng/ml & 50 ng/ml)	IFN-γ (500 U/ml)	IFN-γ, IL-1β, TNF-α (20 ng/ml)
Quality of study (ARRIVE score)	High (89)	High (75)	High (75)	High (75)	High (75)	High (75)

MS, multiple sclerosis; EAE, experimental autoimmune encephalomyelitis; MOG, myelin oligodendrocyte glycoprotein; NR, not reported; hUCMSCs, human umbilical cord mesenchymal stem cells; HLA, human leukocyte antigen; IV, intravenous; OPC, oligodendrocyte progenitor cell; ARRIVE, Animal Research: Reporting of In Vivo Experiments.

Anat Cell Biol 2024;57:333~345 https://doi.org/10.5115/acb.24.082