pISSN 2093-3665
eISSN 2093-3673
pISSN 2093-3665
eISSN 2093-3673
The growth factors, endpoints, sources and cell types, melatonin as a supplement, in spermatogonial stem cells freezing medium
| Source | Cell in freezing medium | GF culture | Endpoint | Reference (yr) |
|---|---|---|---|---|
| Mice | Testes | None | Melatonin as an antioxidant increases viability of SSCs by reducing oxygen free radicals and induces apoptosis in damaged cells. | Gholami et al. (2014) [24] |
| Goat | SSCs | FBS, GDNF | 1. Melatonin has an anti-apoptotic function. By regulating Bax-Bcl2 gene expression and caspase-3. 2. Melatonin has an antioxidant function by stimulating the secretion of SOD, CAT, and GSH-Px enzymes. 3. Melatonin has an anti-autophagy function by regulating the secretion of LC3-I, LC3-II, P62, Beclin1, and ATG7 proteins. |
Feng et al. (2020) [27] |
| Mice | SSCs | FBS, GDNF, bFGF, LIF | Melatonin as an antioxidant improve viability of SSCs by reducing oxygen free radicals and improves transplanting SSCs into adult male into mature azoospermic mice. | Kazemzadeh et al. (2022) [28] |
| Mice | SSCs | FBS, GDNF, bFGF, LIF | Melatonin as an antioxidant and anti-apoptotic can be useful in the long-term preservation of SSCs in cryogenic environment. | Nazeri et al. (2022) [29] |
GF, growth factor; SSC, spermatogonial stem cell; FBS, fetal bovine serum; GDNF, glial cell line-derived neurotrophic factor; SOD, superoxide dismutase; CAT, catalase; GSH-Px, glutathione peroxidase; LC3, light chain 3; bFGF, basic fibroblast growth factor; LIF, leukemia inhibitory factor.
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