Fig. 2. Micrographs of hematoxylin and eosin-stained rats’ ovarian sections (A–E). (A) Ovarian sections in the control group illustrate multiple growing GF, CL, vascularized M, PR with observable ovum. (B, C) The SOF-treated ovary demonstrating atretic follicile (T), congested B and multiple V. (D) The Vit E-treated ovary illustrates healthy follicles with multiple corpora lutea. (E) The SOF+Vit E co-treated ovary demonstrates health primary follicles with multiple corpora lutea and mildly vascularized medulla. (F–H) Histograms illustrate the morphometric study of the effect of SOF and Vit E on treated rats’ ovaries: (F) healthy follicles. (G) Atretic follicile and (H) corpora lutea. The SOF-treated group illustrates a significant reduction in the number of atretic follicles and corpora lutea compared to the control. While the number of atretic follicles was significantly increased in the SOF group compared to the control, *P<0.01, **P<0.001, ****P<0.00001. In the SOF+Vit E co-treated group, the number of healthy follicles and corpora lutea are significantly upregulated, while the atretic follicles are significantly downregulated, ####P<0.00001. One-way ANOVA was used to compare between multiple groups, unpaired t-test was used to compare between two groups. Scale bar: 50 μm. GF, graafian follicles; CL, corpus luteum; M, medulla; PR, primary follicle; SOF, Sofosbuvir; B, congested blood vessles; V, vacuolations; Vit E, vitamin E.
© Anat Cell Biol
pISSN 2093-3665
eISSN 2093-3673
