Fig. 3. H&E staining. (A, B) Three days after the cell culture, only mesenteric decellularized tissue was observed (magnification: A, ×100 and B, ×200). (C, D) Cells on day 10 at the site of interaction between blastema tissue and mesenteric extracellular matrix (ECM) are observed (magnification: C, ×200 and D, ×400). (E, F) The cell polarity is shown at the site of interaction between blastema tissue and the mesenteric matrix on day 15 (magnification: E, ×200 and F, ×400). (G, H) Cell migration indicates blastemal cells, along with the mesenteric matrix and proliferating cells at this site on day 21 (magnification: G, ×200 and H, ×400). Arrows show adherence and maintenance of blastema cells in the ECM scaffold during culture after the 10th day.
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