Fig. 2. Schematic overview of the preparation, assembly, and co-culture of the mesenteric ECM and blastema tissue ring. SDS and triton X-100 solutions decellularize the mesenteric tissue. As well as, to prepare blastema tissue, the New Zealand rabbit’s ears were punched, and two days after punching the ears, the blastema ring derived from the periphery of the primary hole. Next, the blastema rings and the ECM scaffolds were assembled and co-cultured in a medium containing fibronectin. ECM, extracellular matrix; SDS, sodium dodecyl sulfate.
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