Fig. 8. Increase in flip-flops following 30-minute nisin treatment. (A) Green fluorescence of annexin V induced by nisin indicates flip-flop. Fluorescence and bright field (BF) microscopy images in unfixed cells. Scale bar=20 μm. (B) Q uantitative analysis of flip-flops. When index of the image is 100, it means that the ratio of the annexin V positive cell is the same as the average ratio in the control images. (C) Viability after 30-minute nisin treatment or 24-hour after treatment with the apoptosis inducer, staurosporine. The line within the box plot represents the median value. Values are expressed as the mean±SD (n>15). *P<0.05 versus control.
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