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Fig. 1
Fig. 1Actin polymerization inhibitor cytochalasin (cyto)-D suppresses nisin-induced spherical distribution composed of cytokeratin (CK) 5 and CK17. Interspacing between CK17 intermediate filaments (asterisk) are presented as a 2D structure and it is different from the 3D spherical distribution in . This interspacing could only be observed in the X-Y plane. In the X-Z plane or Y-Z plane, the interspacing observed in the X-Y plane did not exist. (A–F) 3D images consist of stacked super resolution microscopy images (green) and fluorescence microscopy (blue) along the z-axis. Control conditions shown in panels (A) and (D) are with the same data as that in Fig 1A no. 3 and 7. (G) Cut surface of (F). Upper left window is the white line height section of the lower window. Upper left window can also be explained as the red line height section of the right window. Two arrows at right lower corner of each window indicate the axis of each plane. Panel (G) have been rotated approximately 45 degrees counterclockwise compared to (F). The meaning of each indicator is illustrated. Scale bars represent 10 μm. *Interspaces between CK17 intermediate filaments.
Anat Cell Biol 2022;55:190~204
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